Sedentary Behavior Interrupted: A Trial of Acute Effects on Biomarkers of Healthy Aging

Open to females ages 55 years and up

• female
• 55 years of age and above
• any ethnicity or race
• screened to sit for 8 or more hours per day, perform less than 60 sit-to-stand transitions per day & engage in less than 20 minutes of moderate-to-vigorous physical activity (MVPA) per day
• BMI 25 - 45 kg/m2
• ambulatory
• medically stable without any health conditions that would inhibit standing or PA;
• able to give informed consent & comply with study protocols;
• able to read, communicate, and write fluently in English;
• able to travel to study visits;
• no menstruation for at least one year;
• not participating or not planning to participate in a physical activity or weight loss program or research study.

• Mental state that would preclude complete understanding of the protocol or compliance;
• type 1 diabetes;
• type 2 diabetes using insulin or with poor glycemic control (greater than 10% for all participants);
• poorly controlled hypertension (Systolic Blood Pressure ≥165 or Diastolic Blood Pressure ≥100);
• chronic illness that may be associated with weight change (HIV/AIDS, active cancer, or uncontrolled thyroid disease),
• anemia (hemoglobin ≤11g/dL);
• personal or first-degree relative history of venous thrombosis;
• weight instability in past 3 months (no more than 5% up or down);
• regular use of vasodilator medication and high risk of stroke and/or heart attack (i.e., history of multiple hospitalizations (>2X) in the last 6 months), congestive heart failure, atrial fibrillation, and/or stroke;
• regular use of immunosuppressant or corticosteroid medication;
• blood donation less than 56 days prior to screening visit,
• smoking cigarettes or smoking or ingesting anything, and other use of tobacco products including e-cigarettes.
• diagnosis of orthostatic hypotension